Protein_Domain
Part:BBa_K2401005:Design
Designed by: Shanti Ricke Group: iGEM17_Hamburg (2017-10-20)
Ybt and Pch inner membrane siderophore transporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The siderophore transport systems should be used to create siderophore therapy testing strains. Therefore, we had to make sure that we utilize two different inducing mechanisms because we want to test one siderophore at the time.
Source
Genomic Sequences of klebsiella pneumonia and pseudomonas aeruginosa